Volume 6, Number 12-14, January - December, 2014

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About the Cover

Appropriate detection of Carbapenem Resistant Enterobacteriaceae (CRE) is vital for patient care in order to institute correct therapeutic options. Molecular techniques can easily differentiate classes but the need of the hour is a rapid practical phenotypic method which can differentiate KPCs and MBLs in Enterobacteriaceae. The present study detects and differentiates class A and B carbapenemases among Enterobacteriaceae by simple phenotypic methods that uses both inhibitors EDTA and Phenyl Boronic Acid. Among 38 CRE isolated from 447 strains of Enterobacteriaceae 14 showed class B (MBL) and none showed class A (KPC) carbapenemases phenotypically. 19 were positive for MBL genes and none for KPC genes by multiplex PCR. To conclude, this phenotypic method is very helpful to detect carbapenemase production and provides a simple algorithm for the differentiation of Class A carbapenemases and MBL enzymes in Enterobacteriaceae in routine clinical microbiological laboratories (Ref: Savitha Hiremath, Renushri BV, Nagaraj ER. Phenotypic differentiation of class A and class B Carbapenemases in Enterobacteriaceae. Disease, 2014, 6(13), 13-17), (Image: www.bioquell.com).

ANALYSIS

Efficacy of vamana followed by nyagrodadi churna in the management of sthula madhumeha (NIDDM)

Meera KJ, Pujar P Muralidhar

The present study is designed to assess the efficacy of Vamana, one of the detoxification treatment procedures of Ayurveda followed by administration of oral herbal medicine in Sthula Madhumeha with special reference to Noninsulin dependent Diabetes Mellitus (NIDDM). The prevalence of the disease Diabetes Mellitus is increasing all over the world. Shodana (Detoxification procedure) followed by shamana (Oral medication) is the treatment measure for Sthoola Madhumeha as explained in Ayurvedic text books. Thus the present study was undertaken to assess the effective management of NIDDM from classical ayurvedic point of view i.e., Vamana followed by NygrodadiChurna. The Screening of the patients between the age group of 30 to50 years was done randomly and finally 30 patients who fulfilled all necessary criteria and gave a written consent for the clinical trial were registered for the study. The selected patients were assessed for both subjective and biochemical parameters before and after treatment schedule. The study showed significant reduction in blood sugar level (P<0.001, ANOVA) after the treatment schedule.

Disease, 2014, 6(12), 6-9

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ANALYSIS

Phenotypic differentiation of class A and class B Carbapenemases in Enterobacteriaceae

Savitha Hiremath, Renushri BV, Nagaraj ER

Appropriate detection of Carbapenem Resistant Enterobacteriaceae (CRE) is vital for patient care in order to institute correct therapeutic options. Molecular techniques can easily differentiate classes but the need of the hour is a rapid practical phenotypic method which can differentiate KPCs and MBLs in Enterobacteriaceae. The present study detects and differentiates class A and B carbapenemases among Enterobacteriaceae by simple phenotypic methods that uses both inhibitors EDTA and Phenyl Boronic Acid. Among 38 CRE isolated from 447 strains of Enterobacteriaceae 14 showed class B (MBL) and none showed class A (KPC) carbapenemases phenotypically. 19 were positive for MBL genes and none for KPC genes by multiplex PCR. To conclude, this phenotypic method is very helpful to detect carbapenemase production and provides a simple algorithm for the differentiation of Class A carbapenemases and MBL enzymes in Enterobacteriaceae in routine clinical microbiological laboratories.

Disease, 2014, 6(13), 13-17

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CASE REPORT

Management of Anterior Maxillary Radicular Cyst

Subba Raju T, Sita Rama Kumar M

The radicular cyst is the most common inflammatory odontogenic cystic lesion of the jaws. It usually originates as a sequel to a periapical inflammatory process, following chemical, physical or bacterial injury. Due to its chronic etiology, the cyst usually appears towards the later stage of life. It has a male sex predilection, with the maxillary anterior region as the most common site of involvement. In this article, a case report of radicular cyst with treatment is discussed in regard to current literature.

Disease, 2014, 6(14), 21-23

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