Volume 6, Number 17, Pages 38-56, July - September , 2015

Table of Contents

Previous | Next

About the Cover

The present study was intended to find out novel drug formulations of commercial antibiotics using chitin and chitosan against an oral pathogen Streptococcus mutans. Chitin was derived from shrimp shell waste and chitosan was prepared from the same. These compounds and commercially available antibiotics such as amoxicillin and ciprofloxacin were used to find out antibacterial sensitivity. Amount of chitosan derived from chitin was estimated by colorimetric method and which was estimated as 95mg/ml. Antibiotic sensitivity assay using modified Kirby Bauer method was followed to find out antibacterial sensitivity and result showed no inhibitory effect for chitin and the chitosan and amoxicillin were moderately sensitive. Synergistic effects of chitin and chitosan combined with commercial antibiotics were studied and the combinatorial applications were highly sensitive to the dental carries pathogens. MIC for the antibiotics and the combinations of chitin, chitosan with antibiotics were determined and MIC for combination of antibiotics and chitosan were determined as 25mg/ml. Insilico prediction based on crystal structure of peptidoglycan is performed. Active Molecular Docking studies using Autodock software reveals Chitosan is potential inhibitor of peptidoglycan molecule of Gram positive Bacteria. Based on these results we can conclude that if the bacteria show resistant to amoxicillin, it can be combined with chitosan in order to increase its sensitivity because chitosan shows higher binding energy and different binding sites. Therefore the combination of the commercial antibiotics with chitosan can be used for therapeutic applications, which may serve as a facilitator to improve the efficiency of commercial antibiotics (Ref: Elizabeth Rani C, Vinoth M, Arunkumar S. Synergistic effects of Chitin & Chitosan derived from shrimp waste with commercial antibiotics against Streptococcus Mutans. Discovery Biotechnology, 2015, 6(17), 38-51).

RESEARCH

Synergistic effects of Chitin & Chitosan derived from shrimp waste with commercial antibiotics against Streptococcus Mutans


Elizabeth Rani C, Vinoth M, Arunkumar S

The present study was intended to find out novel drug formulations of commercial antibiotics using chitin and chitosan against an oral pathogen Streptococcus mutans. Chitin was derived from shrimp shell waste and chitosan was prepared from the same. These compounds and commercially available antibiotics such as amoxicillin and ciprofloxacin were used to find out antibacterial sensitivity. Amount of chitosan derived from chitin was estimated by colorimetric method and which was estimated as 95mg/ml. Antibiotic sensitivity assay using modified Kirby Bauer method was followed to find out antibacterial sensitivity and result showed no inhibitory effect for chitin and the chitosan and amoxicillin were moderately sensitive. Synergistic effects of chitin and chitosan combined with commercial antibiotics were studied and the combinatorial applications were highly sensitive to the dental carries pathogens. MIC for the antibiotics and the combinations of chitin, chitosan with antibiotics were determined and MIC for combination of antibiotics and chitosan were determined as 25mg/ml. Insilico prediction based on crystal structure of peptidoglycan is performed. Active Molecular Docking studies using Autodock software reveals Chitosan is potential inhibitor of peptidoglycan molecule of Gram positive Bacteria. Based on these results we can conclude that if the bacteria show resistant to amoxicillin, it can be combined with chitosan in order to increase its sensitivity because chitosan shows higher binding energy and different binding sites. Therefore the combination of the commercial antibiotics with chitosan can be used for therapeutic applications, which may serve as a facilitator to improve the efficiency of commercial antibiotics.

Discovery Biotechnology, 2015, 6(17), 38-51

Full Text | PDF

Little finger curvature: a morphogenetic trait inherited by Mendelian pattern among the IGBO ethnic group of Nigeria


Ordu KS, Nwosu NC

The bending inward of the little finger is a trait that is inherited from parents and its inheritance follows a pattern. This study therefore, was conducted to investigate the pattern of inheritance of the little finger curvature amongst the Igbo ethnic group of Nigeria using 1022 subjects from 315 families with 630 parents and 392 offspring were Igbos. Both couple in a family, their parents and grandparents were of Igbo origin. The little finger curvature was observed physically in the father, mother, and at least a child in each family and recorded. The results were analysed by testing the significance of the observed values to the expected values. On the assumption that offspring outcome conforms to Mendelian simple dominant-recessive monohybrid cross. Statistically chi-square test at p<0.05 was used for the test and the inheritance pattern of the little finger curvature was determined. The results showed that the little finger curvature observed in this study was genetically determined and follows Mendelian single gene dominant-recessive pattern with the allele for curved little finger dominant over the allele for straight little finger. The results that were gotten will be useful in forensic and genetic studies and might also be useful as a Medico-legal tool in settlement of parental dispute.

Discovery Biotechnology, 2015, 6(17), 52-56

Full Text | PDF