Volume 6, Number 16, Pages 16-37, April - June, 2015

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About the Cover

Inula royleana DC. is a threatened medicinal plant belonging to genus Inula, tribe Inuleae and family Asteraceae. It is a rich source of lycoctonine and anthranoyl-lycoctonine alkaloids. Sesquiterpene lactones of eudesmane type, abietane diterpenes and diterpene alkaloids are also reported from its roots. This plant is also used traditionally to cure various diseases like headache, dermatitis, allergy, intestinal problems, lowering hypertension etc. This plant has ethnoveterinary importance as well where it is used to cure wounds and inflammation of hooves. During present study efficient in vitro propagation strategies have been developed for I. royleana by using different explants (leaf, petiole, involucral bracts and seeds). MS medium was supplemented with different growth hormones both individually and in different combinations but MS medium containing BAP(5mg/l) and IAA(2mg/l) in combination proved to be most effective in inducing maximum callus and regenerating 7.6±0.49 mean number of shoots with mean length of 6.2±0.28cm from leaf explant. Regenerated shoots produced 4±0.2 mean number of roots on MS medium supplemented with IBA (0.8mg/l). These in vitro plantlets were successfully acclimatized within 4 weeks with 100% survival rate (Ref: Samar Amin, Zahoor A Kaloo, Seema Singh. In vitro propagation strategies for ex- situ conservation of Inula royleana DC., a threatened medicinal plant of Kashmir Himalaya. Discovery Biotechnology, 2015, 6(16), 16-29), (Image: www.chadwellseeds.co.uk).

ANALYSIS

In vitro propagation strategies for ex- situ conservation of Inula royleana DC., a threatened medicinal plant of Kashmir Himalaya


Samar Amin, Zahoor A Kaloo, Seema Singh

Inula royleana DC. is a threatened medicinal plant belonging to genus Inula, tribe Inuleae and family Asteraceae. It is a rich source of lycoctonine and anthranoyl-lycoctonine alkaloids. Sesquiterpene lactones of eudesmane type, abietane diterpenes and diterpene alkaloids are also reported from its roots. This plant is also used traditionally to cure various diseases like headache, dermatitis, allergy, intestinal problems, lowering hypertension etc. This plant has ethnoveterinary importance as well where it is used to cure wounds and inflammation of hooves. During present study efficient in vitro propagation strategies have been developed for I. royleana by using different explants (leaf, petiole, involucral bracts and seeds). MS medium was supplemented with different growth hormones both individually and in different combinations but MS medium containing BAP(5mg/l) and IAA(2mg/l) in combination proved to be most effective in inducing maximum callus and regenerating 7.6±0.49 mean number of shoots with mean length of 6.2±0.28cm from leaf explant. Regenerated shoots produced 4±0.2 mean number of roots on MS medium supplemented with IBA (0.8mg/l). These in vitro plantlets were successfully acclimatized within 4 weeks with 100% survival rate.

Discovery Biotechnology, 2015, 6(16), 16-29

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RESEARCH

A comparative genetic diversity study among elite Bt and Non-Bt cotton varieties of Andhra Pradesh by RAPD markers


Chandrashekar R, Bylla Prasad, Narendar Vankudothu, Jyothi Chaitanya P, Lakshmi Bhavani N, Rama Reddy Y, Karunaker Rao Kudle

The present work dealt with RAPD markers for genetic diversity comparison estimation in twenty three cotton varieties of Bt and non-Bt cotton cultivars belonging to two cultivated species of cotton in Andhra Pradesh state, India.Variations among twenty three different cotton varieties were analyzed with selected 40 RAPD primers using the polymerase chain reaction (PCR). All the primers produced polymorphic amplicons; however the extent of polymorphism varied with each primer in twenty three cotton varieties. Out of a total of 409 RAPD bands generated from the twenty three varieties, 257 bands perceived polymorphism. The maximum similarity of 94.6% was observed between Gossypium hirsutum and Bt cotton varieties and minimum similarity of 4.4% was observed between Gossypium arboretum and Bt cotton varieties respectively. Cluster analysis by the unweighted pair group method of arithmetic means (UPGMA) resulted in two broad clusters representing twenty three varieties of cotton in each. All the varieties could be discriminated from one another using combined profiles for 40 primers and the relationship among the Bt and non-Bt cotton varieties under study is presented in the dendrogram.

Discovery Biotechnology, 2015, 6(16), 30-37

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