Volume 5, Number 13, January - March, 2014

Table of Contents

Previous | Next

About the Cover

Indigo is a blue colored dye used from ancient times for dyeing of fabrics, now its use is also extended to food and the pharmaceutical industries. The dye is produced chemically on large scale leading to environmental pollution. Biological route for indigo production could be an alternative for sustainable indigo production. The present study focuses on the biological production of indigo from a Pandorea sp. strain, isolated from oil contaminated garage soil as an effective indigo producer in presence of indole as a substrate and silicone oil as a biphasic organic solvent. The production of pigment was confirmed using thin layer chromatography and UV visible spectrophotometer. Further for effective indigo production the following parameters like concentration of substrateindole, pH, and temperature were optimized under single and two phase systems. When the concentration of substrate- indole, was varied from 1 to 50 mM, the maximum yield of 74.42 % was observed in single phase system at a substrate concentration of 4.686 mg/ ml whereas for two phase system the yield maximized to 91.76 % at a substrate concentration of 2.343 mg/ ml. The effect of temperature and pH on indigo production was also studied from 10 oC to 50oC and pH from 6 to 11. The maximum indigo production was 3.933 mg/ml and 4.850 mg/ml at 40 o C (pH -7) and the production was 3.828 mg/ml and 4.719 mg/ml at pH 7 for single and two phase respectively. The rate of indigo production was also studied at indole concentration of 2.343 g/ L for a biphasic system (Ref: Vaishnavi Unde, Srikanth Mutnuri. Bio-catalytic production of indigo using biphasic organic solvent. Discovery Biotechnology, 2014, 5(13), 3-10), (Image: th01.deviantart.net).

RESEARCH

Bio-catalytic production of indigo using biphasic organic solvent

Vaishnavi Unde, Srikanth Mutnuri

Indigo is a blue colored dye used from ancient times for dyeing of fabrics, now its use is also extended to food and the pharmaceutical industries. The dye is produced chemically on large scale leading to environmental pollution. Biological route for indigo production could be an alternative for sustainable indigo production. The present study focuses on the biological production of indigo from a Pandorea sp. strain, isolated from oil contaminated garage soil as an effective indigo producer in presence of indole as a substrate and silicone oil as a biphasic organic solvent. The production of pigment was confirmed using thin layer chromatography and UV visible spectrophotometer. Further for effective indigo production the following parameters like concentration of substrate-indole, pH, and temperature were optimized under single and two phase systems. When the concentration of substrate- indole, was varied from 1 to 50 mM, the maximum yield of 74.42 % was observed in single phase system at a substrate concentration of 4.686 mg/ ml whereas for two phase system the yield maximized to 91.76 % at a substrate concentration of 2.343 mg/ ml. The effect of temperature and pH on indigo production was also studied from 10 oC to 50oC and pH from 6 to 11. The maximum indigo production was 3.933 mg/ml and 4.850 mg/ml at 40 o C (pH -7) and the production was 3.828 mg/ml and 4.719 mg/ml at pH 7 for single and two phase respectively. The rate of indigo production was also studied at indole concentration of 2.343 g/ L for a biphasic system.

Discovery Biotechnology, 2014, 5(13), 3-10

Full Text | PDF